Matsuda, T., Hinomoto, N., Singh, R.N., Gotoh, T., 2012.
Molecular-based identification and phylogeny of Oligonychus species (Acari: Tetranychidae). Journal of Economic Entomology, 105: 1043-1050.
Abstract
The genus Oligonychus has been morphologically divided into two groups based on the direction of curvature of the aedeagus, includes some morphologically similar species that are difÞcult to distinguish. To develop DNA-based methods for identifying Oligonychus species and to determine the phylogenetic relationships among them,weexamined the cytochrome c oxidase subunit I gene of mitochondrialDNAand the internal transcribed spacer and 28S regions of nuclear ribosomal RNA gene for 17 species. Based on the genetic distances (p-distances) of the three DNA regions, the range of intraspeciÞc divergence was found to be below (and not overlap) the range of interspecic divergence, which allowed the 17 species to be discriminated correctly, consistent with their classiÞcation based on morphology. Phylogenetic trees constructed by neighbor-joining and Bayesian methods clearly showed two clades, consisting of species whose aedeagi curve ventrally and dorsally, respectively. Three Oligonychus species inhabiting gramineous plants formed clearly deÞned subclades.
