Lee, M., Suh, S., Kwon, Y., 1999.
Phylogeny and diagnostic markers of six Tetranychus species (Acarina: Tetranychidae) in Korea based on the mitochondrial cytochrome oxidase subunit I. Journal of Asia Pacific Entomology, 2: 85-92.
Abstract
To understand the evolutionary relationship between six Tetranychus species in Korea Republic, 598 nucleotides of cytochrome oxidase subunit I (COI) were sequenced, analysed. The sequences were extremely rich in A+T (77% on average). Nucleotide differences ranged from 6.7 to 14.9% in a pairwise comparison among species. Nucleotide substitutions based on all pairwise comparisons showed 55% of transversions. The largest genetic distance was found between species T. viennensis [Amphitetranychus viennensis] and T. piercei. The inferred phylogenetic tree indicated that T. urticae, T. cinnabarinus and T. kanzawai were clustered together and showed a sister-group relationship with T. truncatus at the highest bootstrap support. A. viennensis showed the largest genetic distance from all other species. T. piercei, a species recently found in Korea Republic, was also distinct from the group composed of four species, and also from A. viennensis. The restriction sites of Alu I, Dde I and Sau 3A were searched in the sequences of COI. Restriction fragment length polymorphisms (RFLPs) in COI genes and intraspecific variabilities of the three restriction sites in diverse colonies of each species collected from different locations and various host plants are shown
