This study aimed to evaluate the effectiveness of the predatory mites Amblydromalus limonicus (Acari: Phytoseiidae) and Stratiolaelaps scimitus (Acari: Laelapidae) in controlling thrips and reducing the incidence of tospoviruses in lettuce crops, considering their potential as biological control agents in integrated pest management programs. The experiment was conducted in a commercial lettuce field located in Biritiba-Mirim, state of São Paulo, Brazil, during February and March 2025. Thrips are considered one of the main pests affecting lettuce production, causing direct feeding damage and acting as vectors of tospoviruses, which can significantly reduce crop yield and quality. In this context, biological control using predatory mites represents a promising alternative for pest management. Three biweekly releases of predatory mites were carried out throughout the crop cycle. In each release, 20,000 individuals of S. scimitus were distributed directly onto the soil, targeting pest stages present in this environment, particularly immature stages associated with the soil. Additionally, 2,000 individuals of A. limonicus were released onto the aerial parts of the plants to prey on thrips present on the leaves. The combined use of these two predatory mite species aimed to enhance pest suppression by acting in different microhabitats within the cropping system. Sixth weeks after the first predator release, lettuce plant samples were collected to quantify and identify thrips populations and to assess the incidence of tospovirusin the crop. The average thrips infestation in the predatory mite treatment was 2.33 insects per plant, a lower valuethan that observed in the control area without predator release, where the infestation reached 6.0 insects per plant. In addition to reducing pest populations, the predatory mite treatment also showed a lower incidence of tospoviruses. Consequently, lettuce plants in the treated area exhibited better development, with an average increase of 32% in plant size compared to plants inthe control area. These results demonstrate that the combined use of A. limonicus and S. scimitus can effectively contribute to the suppression of thrips populations and the reduction of virus incidence in lettuce crops. Therefore, the use of these predatory mites represents a viable and sustainable strategy for integrated pest management, helping to reduce dependence on chemical insecticides and supporting more environmentally sustainable agricultural production systems.