Predatory mite communities in European vineyards are dominated by the species belonging to the Phytoseiidae family. Surveys on these mites were carried out in several Italian regions in the 1980s when insecticides were seldom used against berry moths. Flavescence dorée (FD) epidemics in northeastern Italy (first in Veneto region) in the 1990s necessitated insecticide applications against the leafhopper Scaphoideus titanus, the main vector of FD-associated phytoplasma. Investigations showed that the increase in organophosphate use has not caused a clear decline in predatory mite populations. Recently, new FD epidemics were detected in North-eastern Italy and pyrethroids were selected for the control of vectors. Their low selectivity towards predatory mites suggested a reappraisal of this topic. Records on Phytoseiidae collected in vineyards in the Veneto region and identified at the University of Padova in 1983 and 2025 were compared. Additional data from definite viticultural areas (i.e., the Conegliano Valdobbiadene Consortium territory) regarding phytoseiid mites collected in 1993 and 2025 were also compared. The survey carried out at regional level in 1983 showed that the most common species were Amblyseius andersoni (39.4% of vineyards), Kampimodromus aberrans (24.2%) and Typhlodromus pyri(15.2%). Euseius finlandicus was also found (15.2%) but only in vineyards not treated with insecticides. The survey conducted in 2025 revealed a clear dominance of K. aberrans (accounted for 56.8% of total individuals and recorded in 56.3% of vineyards), followed by A. andersoni(24.2% and 62.7%, respectively) and Phytoseius finitimus Ribaga (15.4% and 27.8%, respectively). Typhlodromus pyri was rarely detected (0.67% and 0.63%, respectively). The survey conducted in the Conegliano Valdobbiadene Consortium area, in 1993, showed that T. pyri was the most frequent species (75% of vineyards) followed by Paraseiulus talbii (29%) and K. aberrans (28%). However T. pyri was dominant in the 65% of vineyards while K. aberrans and T. talbii in 18% and 2% of them. Phytoseiid assemblage in 2025 was markedly dominated by K. aberrans (85.34% of individuals; 87.5% of vineyards), whereas A. andersoni ranked second but at substantially lower abundance and occurrence (11.46% and 37.5%, respectively). Typhlodromus pyri was present in only one vineyard in this survey (3.16% and 2.5%, respectively). The findings suggest substantial changes in phytoseiid communities over the past 40 years, likely due to climate change and, to a lesser extent, insecticide use.