Histiostoma globotuberum sp. n. was collected from Kyoto and Tsukuba, Japan. This species showed one pair of ring or disk like structures (coxal tuber) on the anterior lateral part of coxa I of the deutonymph, adult male and female. Hexane extracts (possibly the opisthonotal gland extract) of the species consisted of 3 hydroxybenzene 1,2 dicarboaldehyde as a major component, in addition to geranial which commonly occurs in histiostomatid mites. Sexual dimorphism was observed in hydrocarbon profiles of mite extracts.