Van Impe, G. van, Legrand, G., 1980.
Practical problems of laboratory assays with acaricides against Tetranychus urticae Koch (Acari: Tetranychidae). Mededelingen van de Faculteit Landbouwwetenschappen Rijksuniversiteit Gent, 45: 691-700.
Abstract
Owing to the wide food-plant range of Tetranychus urticae Koch, new acaricides have frequently to be tested against it. Examples are given for improvements to the laboratory techniques used for the bioassay of such acaricides with this mite. These improvements relate to rearing, especially the alternation of conditions simulating winter, summer temperatures, humidity, photoperiod and light intensity (instead of the usual continuous provision of summer conditions, which in time reduces fecundity and does not eliminate diapause), the use of an aspirator (which is described) to transfer mites, an improved apparatus for spraying the mites with the test acaricide, an improved device for maintaining treated mites, and a flotation technique for keeping leaf-discs of their food-plants fresh, the measurement of response to a compound by a single criterion such as actual mortality, in order to eliminate subjective interpretations of varied terms, and the statistical methods used to interpret and report results, with the circumstances most suitable for the application of each
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