The seasonality of Amblyomma triguttatum triguttatum was investigated using carbon dioxide traps set up in Durikai State Forest, Queensland, Australia, and by counting the number of the different tick stages found on the ears of Macropus giganteus. Larvae of A. t. triguttatum were found from March to September; they were more abundant in March-April. Nymphs were detected from March to December with the peak number during August. Adult ticks were found from November to March with the maximum number in December-January. The sequence of the peak numbers of the different tick stages substantiates the assumption that a single generation of A. t. triguttatum is produced yearly. A forested habitat supported a larger tick population than an adjacent open pasture. One hundred seventy-seven (69 %) of the 258 M. giganteus ears were infested with A. t. triguttatum which was the only tick species found on them. Larvae, nymphs adults of Haemaphysalis bancrofti were also caught in the traps set up in the forested habitat.